Colloidal graphenes as heterogeneous additives to enhance protein crystal yield.

نویسندگان

  • Benjamin S Gully
  • Jianli Zou
  • Gemma Cadby
  • Daniel M Passon
  • K Swaminathan Iyer
  • Charles S Bond
چکیده

In the structural analysis of proteins via X-ray diffraction, a rate-limiting step is in favourable nucleation, a problematic obstacle in successful generation of protein crystals. Here graphene and graphene oxide were applied to protein crystallisation trials, offering improvements in crystalline output and nucleation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Optimizing refolding condition for recombinant tissue plasminogen activator

Low molecular size additives such as L-arginine and the redox compounds have been used both in the culturemedium and in vitro refolding to increase recombinant proteins production. Additives increase proteinrefolding and yield of active proteins by suppressing aggregate formation or enhancing refolding process.In this work, a comparative study was performed on refolding of rec...

متن کامل

Enhancing the Photocatalytic Activity of Nano Nd-TiO2@SiO2 by coupled systems

Metal doped TiO2 catalysts were synthesized by sol-gel method and their activities were determined for degradation of methyl orang as a pollutant. Nd-doped TiO2 showed the maximum activity comparing with others. Colloidal SiO2 was used as a support to increase the surface area and also the activity. Nano-TiO2/SiO2/Nd was synthesized by sol-gel method and it was characterized using XRD, XRF, SEM...

متن کامل

Screening and separation of beta-lactam antibiotics using protein-engineered enzymes.

With recent advances in biotechnology, it is now possible to synthesize a variety of valuable biologically active products from microbial sources. However, screening and economic recovery of these biosynthetically derived products poses a complex problem. The whole culture broth is an extremely heterogeneous aqueous mixture of biomass, nutrients, residual substrates, and other additives. In som...

متن کامل

سنتزتوده‌های کروی TS-1 با استفاده از رزین با قابلیت تبادل آنیون به عنوان درشت‌قالب‌گیر

for several recent years, syntheses of spherical zeolitic materials, such as S-1, ZSM-5 and Beta, have been reported using an anion exchange resin as macro-templates. Conceptually, these syntheses are based on the use of a colloidal crystal templating process. The advantage of this method is preparation of pure self-bonded zeolite micro/macrostructures. In this paper the synthesis of TS-1 is...

متن کامل

Towards graphene iodide: iodination of graphite oxide.

Halogenated graphene derivatives are interesting owing to their outstanding physical and chemical properties. In this paper, we present various methods for the synthesis of iodinated graphene derivatives by the iodination of graphite oxides prepared according to either the Hummers or Hofmann method. Both graphite oxides were iodinated by iodine or hydroiodic acid under reflux or in an autoclave...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Nanoscale

دوره 4 17  شماره 

صفحات  -

تاریخ انتشار 2012